Preparation, Modification and Characterization of Sputtered Carbon Surfaces for Their Application in Biosensor Arrays
(Room Room 104-C)
02 May 19
10:40 AM
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11:00 AM
Tracks:
Coatings and Processes for Biomedical Applications
We describe the chemical modification of carbon surfaces with an eye towards employing them in biosensor (DNA) arrays. Carbon was deposited in thin film form by DC and HIPIMS magnetron sputtering. These depositions were confirmed by atomic force microscopy step height measurements. As indicated by X-ray photoelectron spectroscopy (XPS), the resulting material contained some oxidized carbon at its surface, including –COOH type moieties. These groups could be directly activated for amine attachment using a combination of a carbodiimide (EDC) and sulfo -N-hydroxysuccinimide. Direct attachment of a variety of amines was then possible on this surface, where this process could be followed through the N 1s XPS signal. The surfaces were also activated by direct chlorination using PCl5, where this process could again be followed by XPS – XPS revealed the introduction and disappearance of chlorine. That is, the chlorine on the resulting carbon surfaces could be nucleophilically replaced with a variety of amines. The DC and HIPIMS sputtered carbon films showed different numbers of reactive functional groups, and the HIPIMS carbon shows unusually smoothness and density by scanning electron microscopy.